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Induction of murine CD5 expression by v-H-ras
1Department of Microbiology, Michigan State University, East Lansing 48824-1101, USA.
Immunology
|January 1, 1997
Summary
The v-H-ras oncogene can induce CD5 expression on murine B-lineage cells, suggesting CD5 expression is modulated by signaling and lineage commitment. This finding impacts understanding of B-cell development.
Area of Science:
- Immunology
- Molecular Biology
- Oncology
Background:
- The CD5 surface antigen is a common marker on B-lineage cell lines derived from murine bone marrow infected with v-H-ras retroviruses.
- CD5+ B cells are typically absent in adult murine bone marrow, raising questions about their origin or the oncogene's effect.
Purpose of the Study:
- To investigate whether the v-H-ras oncogene induces CD5 expression on B-lineage cells.
- To explore the potential modulation of CD5 expression by specific signaling pathways and early lineage commitment.
Main Methods:
- Generation of B-lineage cell lines from murine bone marrow infected with retroviruses expressing v-H-ras.
- Analysis of CD5 expression on established cell lines.
- Measurement of CD5 mRNA levels.
Main Results:
- v-H-ras was found to induce CD5 expression on two CD5+ pre-B-cell lines derived from murine bone marrow.
- This induction of CD5 expression correlated with elevated steady-state levels of CD5 mRNA.
Conclusions:
- The v-H-ras oncogene can modulate CD5 expression in B-lineage cells.
- These findings suggest that CD5 expression is potentially regulated by specific signaling events and early B-cell lineage commitment.