Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pristane-induced arthritis is CD4+ T-cell dependent

L M Stasiuk1, M Ghoraishian, C J Elson

  • 1Department of Pathology and Microbiology, University of Bristol, UK.

Immunology
|January 1, 1997
PubMed
Summary

Pristane-induced arthritis in mice requires CD4+ T cells. These cells, activated by environmental antigens like heat shock protein 65, play a key role in arthritis development and progression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A comparison of smartphone and infrasound microphone data from a fuel air explosive and a high explosive.

The Journal of the Acoustical Society of America·2024
Same author

IL-1ra polymorphisms and risk of epidural-related maternal fever (EPIFEVER-2): study protocol for a multicentre, observational mechanistic cohort study.

International journal of obstetric anesthesia·2022
Same author

Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor.

Scientific reports·2015
Same author

Autoimmunity and the diverse pathways to B-cell unresponsiveness.

Immunology today·2014
Same author

Systemic gene transfer of binding immunoglobulin protein (BiP) prevents disease progression in murine collagen-induced arthritis.

Clinical and experimental immunology·2014
Same author

Oncocytic choroid plexus carcinoma: case report.

Clinical neuropathology·2009

Area of Science:

  • Immunology
  • Autoimmunity
  • T cell immunology

Background:

  • Pristane-induced arthritis (PIA) is a murine model of autoimmune disease.
  • The role of specific immune cell populations in PIA pathogenesis is not fully understood.

Purpose of the Study:

  • To elucidate the role of CD4+ T cells in the development of pristane-induced arthritis.
  • To investigate the involvement of heat shock protein 65 (hsp 65) in PIA.

Main Methods:

  • Induction of arthritis in mice using pristane.
  • Adoptive transfer experiments using spleen cells.
  • Assessment of T cell proliferation in response to hsp 65.
  • Major histocompatibility complex (MHC) class II restriction analysis.

Related Experiment Videos

Main Results:

  • Pristane-induced arthritis development is dependent on CD4+ T cells.
  • CD4+ T cells, but not CD8+ T cells or B cells, are required for disease reconstitution.
  • T cells reactive to hsp 65 are involved, with CD4+ T cell depletion reducing proliferation.
  • T cell responses to hsp 65 are MHC class II restricted.

Conclusions:

  • Pristane-induced arthritis is a CD4+ T-cell mediated disease.
  • Immunodominant environmental antigens, such as hsp 65, activate pathogenic or protective CD4+ T cells in PIA.