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Related Concept Videos

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

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Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
08:06

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Published on: April 22, 2011

Spatial and temporal expression of cell surface molecules during nephrogenesis

M R Goldberg1, J Barasch, A Shifteh

  • 1Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.

The American Journal of Physiology
|January 1, 1997
PubMed
Summary

Researchers identified new molecules crucial for kidney development by creating antibodies against embryonic kidney cells. One antibody highlighted the glomerular crevice, while another inhibited tubulogenesis, offering insights into kidney formation.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Nephrology

Background:

  • Cell-to-cell interactions are essential for kidney development.
  • Ureteric bud cells induce metanephric mesenchyme differentiation and tubulogenesis.
  • Identifying novel molecules involved in these inductive processes is critical.

Purpose of the Study:

  • To identify new molecules involved in kidney development.
  • To generate monoclonal antibodies (MAbs) targeting surface molecules of induced mesenchymal cells.
  • To characterize MAbs based on antigen expression patterns and functional effects on tubulogenesis.

Main Methods:

  • Generated monoclonal antibodies (MAbs) against rat embryonic day 15 kidney rudiment mesenchymal cells.
  • Screened MAbs for distinctive antigen expression patterns, particularly in the glomerular crevice.
  • Assessed MAbs for functional impact on tubulogenesis, including induction of apoptosis.

Main Results:

  • Identified MAbs that specifically stained the glomerular crevice, the site of glomerular anlage formation.
  • Discovered a MAb that inhibited tubulogenesis by inducing apoptosis in mesenchymal cells.
  • Demonstrated the utility of MAbs in identifying key molecules in kidney development.

Conclusions:

  • This antibody-based approach is effective for discovering novel molecules in kidney development.
  • The identified MAbs and their targets provide new insights into cell-cell interactions and differentiation during nephrogenesis.
  • Further investigation of these molecules will elucidate mechanisms of kidney formation and potential therapeutic targets.