Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method of preparing insects for scanning electron microscopy

J M Bjerke, T P Freeman, A W Anderson

    Stain Technology
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Health education interventions to reduce cannabis and tobacco smoking-related harms among people who use cannabis: a systematic review.

    Health education research·2026
    Same author

    The nature and interpretation of BOLD signals in white matter - A review.

    Magnetic resonance imaging·2025
    Same author

    Whole genome duplication drives transcriptome reprogramming in response to drought in alfalfa.

    Plant cell reports·2025
    Same author

    Polyploidization-driven transcriptomic dynamics in Medicago sativa neotetraploids: mRNA, smRNA and allele-specific gene expression.

    BMC plant biology·2025
    Same author

    Cannabis, cannabinoids and health: a review of evidence on risks and medical benefits.

    European archives of psychiatry and clinical neuroscience·2024
    Same author

    Exploratory Multisite MR Spectroscopic Imaging Shows White Matter Neuroaxonal Loss Associated with Complications of Type 1 Diabetes in Children.

    AJNR. American journal of neuroradiology·2023

    Acidified 2,2-dimethoxypropane (DMP) offers a rapid, effective method for preparing insect specimens, including the sugarbeet root maggot larva, for scanning electron microscopy. This technique avoids morphological changes and simplifies fixation and dehydration processes.

    Area of Science:

    • Entomology
    • Microscopy
    • Specimen Preparation

    Background:

    • Traditional insect fixation and dehydration for scanning electron microscopy (SEM) are time-consuming and can introduce artifacts.
    • The sugarbeet root maggot larva (Tetanops myopaeformis) presents preparation challenges for SEM due to its morphology.

    Purpose of the Study:

    • To evaluate acidified 2,2-dimethoxypropane (DMP) as a superior alternative for preparing insect specimens for SEM.
    • To assess the efficacy of DMP in preserving the morphology of Tetanops myopaeformis.

    Main Methods:

    • Specimens of Tetanops myopaeformis (larvae and adults) were prepared using acidified 2,2-dimethoxypropane (DMP).
    • Morphological integrity of DMP-treated adults was compared to fresh (unfixed) and conventionally fixed (glutaraldehyde-osmium tetroxide) adults.

    Related Experiment Videos

  • DMP's effectiveness was assessed based on its ability to facilitate SEM imaging and preserve fine structures.

    • Main Results:

    • Acidified DMP was the only successful method for preparing Tetanops myopaeformis larvae for SEM.
    • No significant morphological alterations were observed in DMP-treated adult specimens compared to unfixed and conventionally fixed controls.
    • The DMP method demonstrated rapid penetration of water-impermeable cuticles.

    Conclusions:

    • Acidified 2,2-dimethoxypropane (DMP) is a highly effective and efficient alternative for SEM specimen preparation in entomology.
    • DMP simplifies the fixation and dehydration process, saving time and steps while preserving specimen morphology.
    • This method has proven successful across various insect groups, highlighting its broad applicability.