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Related Experiment Videos

Degradable bisphosphonate-alkaline phosphatase-complexed hydroxyapatite implants in vitro

H Denissen1, E van Beek, T van den Bos

  • 1Department of Oral Function and Implantology, Academic Center for Dentistry Amsterdam (ACTA), The Netherlands.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|February 1, 1997
PubMed
Summary

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This study shows that hydroxyapatite implants with bisphosphonate and alkaline phosphatase can degrade controllably. These implants release agents to inhibit bone resorption while promoting bone growth, aiding alveolar bone preservation.

Area of Science:

  • Biomaterials Science
  • Dental Implantology
  • Bone Regeneration

Background:

  • Alveolar bone loss after tooth extraction poses challenges for dental implant success.
  • Degradable hydroxyapatite (HA) implants offer potential for bone regeneration.
  • Combining HA with resorption inhibitors and mineralizing agents may enhance bone maintenance.

Purpose of the Study:

  • To investigate the surface properties and degradation behavior of bisphosphonate (PCP)-alkaline phosphatase (ALP)-complexed HA implants in vitro.
  • To assess the controlled release of PCP and ALP from HA implants.
  • To evaluate the potential of these implants for alveolar bone preservation.

Main Methods:

  • In vitro analysis of PCP-ALP-complexed HA implant surface chemistry and morphology.

Related Experiment Videos

  • Assessment of PCP and ALP release kinetics over time.
  • In vitro mouse bone experiments to determine effective PCP concentrations for resorption inhibition.
  • Evaluation of ALP activity in the presence of released PCP.

    • Main Results:

    • PCP adsorption chemically altered the HA surface, reducing subsequent ALP adsorption.
    • HA implants exhibited steady release of PCP (75 days) and ALP (14 days).
    • Implant microstructure changed, suggesting preconditioning for in vivo bonding and degradation.
    • Released PCP levels were sufficient to inhibit osteoclast activity without blocking osteoblast activity.
    • Released ALP promoted bone formation, and controlled HA degradation was observed.

    Conclusions:

    • PCP-ALP-complexed HA implants demonstrate controlled degradation and release of therapeutic agents.
    • The implants can modulate bone resorption and enhance bone formation, supporting alveolar bone maintenance.
    • This concept offers a promising strategy for preserving the edentulous alveolus through temporary scaffolding for bone growth.