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Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based
I Gonzalez1, K A Grant, P T Richardson
1BBSRC Institute of Food Research, Reading Laboratory, Earley Gate, United Kingdom.
Journal of Clinical Microbiology
|March 1, 1997
Abstract:
A PCR method for the rapid identification and discrimination of thermophilic Campylobacter jejuni and Campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceuE). A nucleotide sequence divergence of approximately 13% in the ceuE genes of C. jejuni and C. coli facilitated the design of two species-specific PCR primer sets. The specificity of the PCR amplification reactions was confirmed by using two nonradioactively labelled species-specific internal oligonucleotide hybridization probes for each of these species.