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Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
Dynamics of glucose consumption in yeast
B Sonnleitner1, S A Rothen, H Kuriyama
1Institute for Biotechnology, ETH Zürich, Switzerland. Sonnleitner@TWI.CH
Biotechnology Progress
|January 1, 1997
Summary
Yeast cultures exhibit memory by adapting their metabolic enzymes. This adaptation, involving an intracellular enzymatic pool, allows them to manage changes in glucose supply and dilution rates.
Area of Science:
- Microbiology
- Biochemical Engineering
- Systems Biology
Background:
- Continuously cultured microorganisms adapt to specific environmental conditions.
- Sudden changes in nutrient availability can disrupt metabolic homeostasis.
Purpose of the Study:
- To investigate the reproducible overshoot of residual glucose in yeast cultures.
- To quantitatively model the observed phenomenon using an extended bottleneck model.
Main Methods:
- Continuous yeast culture at a low dilution rate.
- Step-wise increase in dilution rate to challenge the culture.
- Quantitative modeling based on an extended bottleneck model.
Main Results:
- Reproducible overshoot of residual glucose concentration observed upon increased dilution rate.
- Model quantitatively predicted overshoots by incorporating an intracellular enzymatic pool.
- Relaxation time for enzymatic pool increase determined to be approximately 1 hour.
- Enzymatic pool shrinkage rate dependent on the reciprocal of the specific growth rate.
Conclusions:
- Microbial populations possess a memory mechanism.
- Metabolic adaptation, specifically the expansion and contraction of enzymatic pools, allows memorization of recent environmental history.
- This adaptation is crucial for managing fluctuating substrate supply and maintaining metabolic stability.
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