Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A modified fluorescence in situ hybridization (FISH) technique

A S Multani1, V L Hopwood, S Pathak

  • 1Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.

Anticancer Research
|November 1, 1996
PubMed
Summary

We modified the fluorescence in situ hybridization (FISH) technique using microwave or formalin pretreatment, achieving comparable signal intensity to the routine method. Hydrogen peroxide pretreatment, however, completely eliminated FISH signals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Characteristics and Outcomes of Anemia in Critically Ill Patients: A Cross-Sectional Observational Study from a Tertiary Intensive Care Unit in Nepal.

Kathmandu University medical journal (KUMJ)·2026
Same author

The Effect of a Modified Tenodesis Wrist-hand Orthosis on Hand Function in Patients With Tetraplegia.

Canadian prosthetics & orthotics journal·2024
Same author

Evaluating the therapeutic potential of different sources of mesenchymal stem cells in acute respiratory distress syndrome.

Stem cell research & therapy·2024
Same author

Clinical and Endoscopic Findings in Patients Presenting with Upper Gastrointestinal Bleeding at a Tertiary Care Hospital.

Kathmandu University medical journal (KUMJ)·2024
Same author

Postoperative surveillance after surgery for colorectal liver metastasis: a cross-sectional study.

Annals of the Royal College of Surgeons of England·2023
Same author

Role of chest radiograph in predicting the need for ventilator support in COVID-19 patients.

African journal of thoracic and critical care medicine·2023

Area of Science:

  • Molecular Biology
  • Cytogenetics
  • Biotechnology

Background:

  • Fluorescence in situ hybridization (FISH) is a crucial technique for detecting specific DNA sequences in chromosomes.
  • Traditional FISH protocols can be time-consuming and require optimization for specific applications.
  • Accelerating FISH procedures is desirable for increased throughput in research and diagnostics.

Purpose of the Study:

  • To modify and shorten the standard fluorescence in situ hybridization (FISH) technique.
  • To evaluate the efficacy of microwave and formalin pretreatment for chromosome preparation.
  • To assess the impact of hydrogen peroxide pretreatment on FISH signal intensity.

Main Methods:

  • Modified the routine FISH technique using microwave or formalin pretreatment for artificial aging.

Related Experiment Videos

  • Employed microwave heating solely for dehydration of chromosome preparations.
  • Utilized an all-human telomere probe on murine K-1735 melanoma cells.
  • Investigated the effect of hydrogen peroxide (H2O2) pretreatment on FISH signals.

    • Main Results:

    • The modified FISH technique, employing microwave or formalin pretreatment, yielded signal intensities comparable to the routine procedure.
    • Microwave heating for dehydration effectively replaced standard dehydration steps.
    • Pretreatment with hydrogen peroxide (H2O2) completely abolished FISH signals, indicating its detrimental effect.

    Conclusions:

    • Shortened FISH protocols using microwave or formalin pretreatment are effective and maintain signal quality.
    • Hydrogen peroxide pretreatment is incompatible with this modified FISH technique and should be avoided.
    • These modifications offer a faster and potentially more efficient approach to FISH analysis.