Poly(ethylene glycol)-conjugated surfactants promote or inhibit aggregation of phospholipids

The calcium-induced aggregation of dilauroyl phosphatidic acid (DLPA) suspensions, with or without added poly(ethylene oxide) (PEO)-conjugated surfactants containing 4 to 30 ethylene oxide subunits, were monitored by turbidity measurement and quasi-elastic light scattering (QLS). The aggregation was inhibited (protected) by the incorporated PEO surfactant for most samples, while a window for promotive effect was found for samples with low surface coverage by the PEO moiety of the incorporated surfactant. Promotion occurs only when the aggregation is slow and at a low level. The promotion is explained by the synergistic effect of PEO and divalent calcium cations when the steric repulsion is weak. The promotion/protection crossover is a display between the PEO/calcium synergistic effect and the steric repulsion.