Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-function relationship of trichosanthin

Y B Ke1, J K Chen, H L Nie

  • 1Shanghai Institute of Cell Biology, Academia Sinica, China.

Life Sciences
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence of Spin-Interference Effects in Exclusive J/ψ→e^{+}e^{-} Photoproduction in Ultraperipheral Heavy-Ion Collisions.

Physical review letters·2026
Same author

First Observation of Deuteron-Λ Correlations at RHIC.

Physical review letters·2026
Same author

Observation of Charmonium Sequential Suppression in Heavy-Ion Collisions at the Relativistic Heavy Ion Collider.

Physical review letters·2026
Same author

[CT-guided percutaneous polidocanol injection for giant pulmonary bulla: a report of 5 cases].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2026
Same author

Energy Independence of the Collins Asymmetry in p^{↑}p Collisions.

Physical review letters·2026
Same author

Precision Measurement of Net-Proton-Number Fluctuations in Au+Au Collisions at RHIC.

Physical review letters·2025

Trichosanthin (TCS) fragments were generated and tested for activity. Two fragments, TCS-F1 and TCS-F2, retained biological activity, though with reduced potency compared to intact TCS.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Trichosanthin (TCS) is a ribosome-inactivating protein from the Trichosanthes plant.
  • Understanding the structure-activity relationship of TCS is crucial for its therapeutic applications.

Purpose of the Study:

  • To investigate the RNA-N-glycosidase and cytotoxic activities of TCS peptide fragments.
  • To identify the potential active site of TCS.

Main Methods:

  • Limited chymotrypsin digestion of TCS to generate peptide fragments (TCS-F1, TCS-F2, TCS-F3).
  • Assay of RNA-N-glycosidase and trophoblast cytotoxicity of the fragments.
  • Structure-based analysis to predict the active site.

Main Results:

Related Experiment Videos

  • TCS-F1 (72-247) and TCS-F2 (100-247) exhibited biological activity.
  • TCS-F3 (133-247) was found to be inactive.
  • TCS-F1 and TCS-F2 showed reduced potency in cytotoxicity compared to intact TCS, but activity was restored at higher doses.
  • The putative active site of TCS is proposed to be located between residues 110 and 174.

Conclusions:

  • Specific peptide fragments of TCS retain biological activity.
  • The C-terminal region of TCS is essential for its full cytotoxic potency.
  • The identified active site region provides insights into TCS function and potential drug design.