Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered cellular proliferation and mesoderm patterning in Polycomb-M33-deficient mice

N Coré1, S Bel, S J Gaunt

  • 1Centre d'immunologie INSERM-CNRS de Marseille Luminy, France.

Development (Cambridge, England)
|February 1, 1997
PubMed
Summary

The M33 gene is crucial for mouse development, regulating skeletal formation and cell growth. Its absence causes severe malformations and suggests a role in controlling retinoic acid access to Hox gene regulatory regions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Frustrated and Concerned: Understanding Antipathy Towards Clients Who Engage in Nonsuicidal Self-Injury in Australian Mental Health Practitioners.

Clinical psychology & psychotherapy·2025
Same author

Developing a platform to investigate the heterogeneity of outcomes for patients with ovarian cancer.

BMJ open quality·2025
Same author

Controlling beam trajectory and transport in a tapered helical undulator.

Journal of synchrotron radiation·2025
Same author

The metabolic landscape of tetrahydrobiopterin metabolism disorders in the Republic of Ireland.

Molecular genetics and metabolism reports·2025
Same author

Analysis of substantiated welfare investigations in extensive farming systems in Victoria, Australia.

Australian veterinary journal·2024
Same author

Person-specific and pooled prediction models for binge eating, alcohol use and binge drinking in bulimia nervosa and alcohol use disorder.

Psychological medicine·2024

Area of Science:

  • Developmental Biology
  • Genetics
  • Epigenetics

Background:

  • Polycomb-group genes maintain homeotic gene expression in Drosophila.
  • M33 is the murine homologue of Polycomb, a key Polycomb-group gene.
  • Understanding Polycomb-group gene function in mammals is essential for developmental biology.

Purpose of the Study:

  • To investigate the developmental role of the murine Polycomb-group gene, M33.
  • To characterize the phenotypic consequences of M33 loss-of-function in mice.

Main Methods:

  • Homologous recombination in embryonic stem (ES) cells to generate M33-deficient mice.
  • Phenotypic analysis of homozygous M33 (-/-) mice.
  • In vitro cell expansion assays for lymphocytes and fibroblasts.

Related Experiment Videos

Main Results:

  • M33 (-/-) mice exhibit significantly retarded growth.
  • Homeotic transformations of the axial skeleton, sternal, and limb malformations were observed.
  • M33 null mutant cells showed impaired in vitro expansion.
  • Retinoic acid treatment aggravated skeletal malformations in M33 null mutants.

Conclusions:

  • The M33 gene plays a critical role in mammalian development, influencing skeletal patterning and cell proliferation.
  • M33 may function by regulating access to retinoic acid response elements in Hox gene regulatory regions.
  • These findings highlight conserved functions of Polycomb-group genes across species.