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Updated: Aug 14, 2026

Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
Published on: September 14, 2012
Effects of 5HT2 receptor antagonists on responses to potassium depolarization in rat isolated aorta
1Department of Clinical Pharmacology, Royal North Shore Hospital, St Leonards, New South Wales, Australia.
Abstract:
1. In order to determine whether 5HT2 receptor antagonists can modify Ca2+ uptake via voltage-operated Ca2+ channel (VOC) in arterial smooth muscle, a comparative study of the effects of selected Ca2+ uptake blockers and 5HT2 receptor antagonists on K(+)-induced contractions of rat aortic strip was undertaken. 2. The antagonist drugs studied included the Ca2+ uptake blockers verapamil, nifedipine, felodipine, diltiazem and cinnarizine, the 5HT2 receptor antagonists cyproheptadine, ritanserin, mianserin, and ketanserin and the alpha 1-adrenoceptor antagonist prazosin. 3. With the notable exception of prazosin, each of these compounds diminished K(+)-induced aortic responses. 4. The following order of potencies (mean IC50 values in mol/L) was established: felodipine (7.0 x 10(-11)) > nifedipine (4.8 x 10(-9) > verapamil (5.5 x 10(-8)) > cyproheptadine (6.2 x 10(-8)) > diltiazem (4.1 x 10(-7)) > cinnarizine (1.3 x 10(-6)) > ritanserin (1.8 x 10(-6)) > ketanserin (9.0 x 10(-6)) > mianserin (2.0 x 10(-5)). 5. The results suggest that antagonists of 5HT2 receptors can modulate Ca2+ uptake via VOC in rat aorta.
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