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CD45-associated protein is a lymphocyte-specific membrane protein expressed in two distinct forms
K Kitamura1, A Matsuda, S Motoya
1Department of Pathology, Roger Williams Medical Center-Brown University, Providence, RI 02908, USA.
European Journal of Immunology
|February 1, 1997
Summary
CD45-associated protein (CD45-AP) production is independently regulated from CD45. CD45-AP stability decreases without CD45, and two mRNA forms exist, impacting protein translocation.
Area of Science:
- Immunology
- Molecular Biology
Background:
- CD45-associated protein (CD45-AP) is a newly discovered protein interacting with CD45.
- The interaction occurs specifically via their transmembrane segments.
Purpose of the Study:
- To investigate the independent regulation of CD45-AP and CD45.
- To determine the effect of CD45 absence on CD45-AP stability.
- To characterize CD45-AP expression in different leukocyte types and identify distinct mRNA forms.
Main Methods:
- Northern hybridization to analyze mRNA production.
- Western blotting to assess protein half-life.
- Analysis of various leukocyte types for CD45-AP expression.
Main Results:
- CD45-AP and CD45 mRNA production are independently regulated.
- CD45-AP protein exhibits reduced stability in the absence of CD45 in most variants.
- CD45-AP is expressed in T, B, and pre-B cells, but not in plasma cells or myeloid cells.
- Two CD45-AP mRNA forms exist, differing by 12 N-terminal amino acids, affecting ER membrane translocation.
Conclusions:
- CD45-AP and CD45 expression are independently controlled.
- CD45 influences CD45-AP protein stability.
- Distinct CD45-AP mRNA variants contribute to differential protein translocation mechanisms.