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IL-10 production in mouse hepatocytes augmented by TGF-beta
S Ishizaka1, S Saito, M Yoshikawa
1Department of Parasitology, Nara Medical University, Kashihara, Japan.
Cytokine
|November 1, 1996
Summary
Supernatants from liver cells boost antibody production in mice. Transforming growth factor beta (TGF-beta) significantly increases interleukin-10 (IL-10) secretion, enhancing this immune response.
Area of Science:
- Immunology
- Cell Biology
Background:
- Liver cells can influence immune responses.
- Interleukin-10 (IL-10) is a key cytokine in immune regulation.
Purpose of the Study:
- To investigate the effect of liver cell supernatant on antibody production.
- To determine the role of transforming growth factor beta (TGF-beta) and IL-10 in this process.
Main Methods:
- Culture of normal liver cells (Clone 1469) and spleen cells.
- Treatment of spleen cells with TGF-beta.
- Measurement of polyclonal antibody production.
- Detection of IL-10 production and mRNA expression using ELISA, ELISPOT, and PCR.
Main Results:
- Liver cell supernatant enhanced polyclonal antibody production in mouse spleen cells, but not human lymphocytes.
- TGF-beta treatment significantly augmented antibody responses.
- IL-10 production and mRNA expression were detected in Clone 1469 cells.
- TGF-beta increased IL-10 secretion from Clone 1469 cells.
Conclusions:
- Liver cell-derived factors, potentially including IL-10, can enhance humoral immunity.
- TGF-beta plays a role in augmenting IL-10 production and subsequent immune enhancement.