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Molecular cloning and sequence analysis of the mouse protein C inhibitor gene
M Zechmeister-Machhart1, P Hufnagl, P Uhrin
1Department of Vascular Biology and Thrombosis Research, University of Vienna, Austria.
Abstract:
The gene encoding mouse protein C inhibitor (mPCI) was isolated and its nucleotide sequence determined. Alignment of the genomic sequence with that of a cDNA obtained from mouse testis revealed that the mPCI gene (like the human counterpart) is composed of five exons and four introns with highly conserved exon/intron boundaries. It encodes a pre-polypeptide of 405 amino acids, which shows 63% identity with human PCI (hPCI). The putative reactive site is identical to that of hPCI from P5 to P3', suggesting a similar protease specificity. Also the putative heparin binding sites and 'hinge' regions are highly homologous in mouse and hPCI.