Cloning of the cDNA encoding rat Presenilin-1

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T Taniguchi1, T Hashimoto, R Taniguchi

  • 1Hyogo Institute for Aging Brain and Cognitive Disorders, Himeji, Japan.

Gene
|February 20, 1997
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...