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Abstract:
It was shown that serious errors in DNA cytofluorimetry may be due to incorrect illumination of photocathode of the photomultiplier in the cytofluorimeters and non-observance of the requirements to the nuclear structures of the cells under measurement with respect to their chemical integrity and thickness. The methods of the optimal illumination of photocathode in the cytofluorimeters and the criteria of selection of the nuclear structures on slides are considered with the aim of obtaining the objective results of DNA cytofluorimetry.