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Cloning and expression of human cDNA encoding Na+, K(+)-ATPase gamma-subunit
1Korean Research Institute of Bioscience and Biotechnology, KIST, Taejon, South Korea.
Biochimica Et Biophysica Acta
|February 7, 1997
Abstract:
A cDNA clone encoding the Na+, K(+)-Atpase gamma-subunit polypeptide was cloned from a human fetal liver cDNA library. The deduced amino acid sequence of the protein comprised 58 amino acids with a calculated molecular weight of 6400 Da, and showed about 86% homology when compared with those of the bovine, rat and mouse Na+, K(+)-ATPase gamma-subunits published elsewhere. Northern blot analysis showed that the cDNA hybridized to a 0.7 kb mRNA which was expressed in human kidney, pancreas and fetal liver.