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Antioxidant defense system in diabetic kidney: a time course study
R Kakkar1, S V Mantha, J Radhi
1Department of Pathology and Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Life Sciences
|January 1, 1997
Summary
Oxidative stress, indicated by increased lipid peroxides and altered antioxidant enzyme activity, is present early in diabetes. This early kidney susceptibility to oxidative stress may contribute to diabetic nephropathy development.
Area of Science:
- Biochemistry
- Physiology
- Endocrinology
Background:
- Diabetes mellitus complications are linked to oxygen free radicals (OFRs).
- Oxidative stress plays a role in the pathogenesis of diabetic nephropathy.
Purpose of the Study:
- Investigate lipid peroxide levels and antioxidant enzyme activities in the kidneys of diabetic rats.
- Assess the impact of diabetes on oxidative stress markers at various stages.
Main Methods:
- Streptozotocin-induced diabetic rat model.
- Measurement of thiobarbituric acid reactive substances (TBARS) and antioxidant enzymes (superoxide dismutase, catalase, glutathione-peroxidase).
- Kidney tissue analysis at weekly intervals over 6 weeks.
Main Results:
- Increased TBARS levels in diabetic kidneys, particularly at later stages.
- Altered catalase and glutathione-peroxidase activities.
- Significant increase in total and CuZn-SOD activity, with no change in Mn-SOD.
Conclusions:
- Oxidative stress is an early feature of diabetes mellitus.
- Kidney susceptibility to oxidative stress in early diabetes is crucial for diabetic nephropathy development.