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A modified confocal laser scanning microscope allows fast ultraviolet ratio imaging of intracellular Ca2+ activity
R Nitschke1, S Wilhelm, R Borlinghaus
1Physiologisches Institut der Albert-Ludwigs-Universität Freiburg, Hermann-Herder-Strasse 7, D-79104 Freiburg, Germany.
Pflugers Archiv : European Journal of Physiology
|March 1, 1997
Abstract:
A confocal, ultraviolet laser scanning microscope (LSM) for reliable ratio measurements of localized intracellular Ca2+ gradients using the Ca2+-sensitive dye Fura-2 was developed. In a commercial LSM, the filter wheels for the excitation band-pass filters and the grey filters were replaced by acousto-optic tunable filters (AOTF) for rapid switching (