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The cowpea mosaic virus RNA 1-encoded 112 kDa protein may function as a VPg precursor in vivo
S A Peters1, J M Mesnard, I M Kooter
1Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.
The Journal of General Virology
|July 1, 1995
Abstract:
Processing of the 112 kDa ('112K') protein encoded by cowpea mosaic virus RNA 1 was examined in cowpea mesophyll protoplasts using a transient expression system. Cleavage of the 112K protein occurred via two alternative pathways either into VPg and 110K (24K + 87K) or into 26K (VPg + 24K) and 87K proteins. The 26K protein can be further cleaved into VPg and 24K proteins. The results support a model in which the 112K protein functions as the precursor of VPg during initiation of replication.