[Cortisol determination in human plasma by a modified method of competitive protein binding]

The authors described a simple and economic modification of the competitive protein binding method of cortisol determination in human blood plasma satisfying the current requirements presented to the saturation analysis. Blood plasma of women given combined contraceptive the preparation was used as a binding system. Analysis of experimental results using a programmed computer was carried out on the basis of standard curve linearization. Preeision sensitivity and specificity of this modified method are assessed. This modification was approved in measuring the cortisol level in the blood plasma of various groups of patients. Comparison with routine competitive binding methods and their analysis is presented.