[Entrapment of antibiotics in liposomes containing phosphatidylethanol]

Antibiotic entrapment to liposomes containing phosphatidyl ethanol, a negatively charged phospholipid, as a lipid component was studied. Antibiotics of various groups such as doxorubicin, rifampicin and doxycycline were entrapped to liposomes consisting of a mixture of phosphatidyl choline and phosphatidyl ethanol at a ratio of 1:1 (v/v) or phosphatidyl choline alone. It was shown that the liposomes containing phosphatidyl ethanol were able to entrap the antibiotics in higher amounts by comparison with the liposomes containing phosphatidyl choline. Phosphatidyl ethanol in the composition of the liposomes had the highest effect on the entrapment of doxorubicin.