Related Experiment Videos
RNA polymerase beta' subunit: a target of DNA binding-independent activation
A Miller1, D Wood, R H Ebright
1Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.
Science (New York, N.Y.)
|March 14, 1997
Abstract:
The bacteriophage N4 single-stranded DNA binding protein (N4SSB) activates transcription by the Escherichia coli final sigma70-RNA polymerase at N4 late promoters. Here it is shown that the single-stranded DNA binding activity of N4SSB is not required for transcriptional activation. N4SSB interacts with the carboxyl terminus of the RNA polymerase beta' subunit in a region that is highly conserved in the largest subunits of prokaryotic and eukaryotic RNA polymerases.