Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in gap junctional intercellular communication in mouse skin carcinogenesis

P R Holden1, B McGuire, A Stoler

  • 1The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Bearsden, Glasgow, UK.

Carcinogenesis
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Airway topicalization in pediatric anesthesia: An international cross-sectional study.

Paediatric anaesthesia·2023
Same author

Design and testing of the safety of the SARUS-CPR hood for novice resuscitators.

Scottish medical journal·2022
Same author

Genetic polymorphism in <i>Methylenetetrahydrofolate Reductase chloride transport protein 6</i> (<i>MTHFR CLCN6</i>) gene is associated with keratinocyte skin cancer in a cohort of renal transplant recipients.

Skin health and disease·2022
Same author

Need for tracheostomy in major head and neck ablative surgery: a paradigm shift during the COVID-19 pandemic.

Annals of the Royal College of Surgeons of England·2022
Same author

Airway management guidance for the endemic phase of COVID-19.

Anaesthesia·2020
Same author

New dimensions in airway management: risks for healthcare staff.

Anaesthesia·2020

Gap junction intercellular communication (GJIC) remains unchanged in early skin cancer stages but significantly decreases in spindle carcinoma cells. E-cadherin loss, not connexin 43, is linked to this communication defect.

Area of Science:

  • Cell Biology
  • Cancer Research
  • Molecular Biology

Background:

  • Gap junction intercellular communication (GJIC) is vital for tissue homeostasis.
  • Dysregulation of GJIC is implicated in various cancers.
  • Chemically induced mouse skin carcinogenesis provides a model to study GJIC alterations during tumor progression.

Purpose of the Study:

  • To investigate changes in GJIC during different stages of mouse skin carcinogenesis.
  • To identify molecular factors associated with decreased GJIC in advanced skin cancer.
  • To explore the role of E-cadherin and connexin 43 in GJIC regulation during tumor progression.

Main Methods:

  • Measurement of GJIC using dye spread assays in various cell lines.
  • Analysis of Ha-ras gene mutations.

Related Experiment Videos

  • Somatic cell hybridization for genetic complementation studies.
  • Western blotting and immunofluorescence to assess protein expression (connexin 43, E-cadherin).
  • Gene transfection to restore E-cadherin expression.

    • Main Results:

    • GJIC levels were similar in normal keratinocytes, papillomas, and squamous carcinomas.
    • A significant 80-90% decrease in GJIC was observed in spindle carcinoma cells compared to squamous carcinoma cells.
    • The defect causing reduced GJIC in spindle cells was recessive and linked to E-cadherin deficiency, not connexin 43 abnormalities.
    • Restoration of E-cadherin partially rescued GJIC in spindle cells.
    • A separate, non-tumorigenic keratinocyte line showed a distinct GJIC defect with abnormal connexin 43 distribution.

    Conclusions:

    • Loss of GJIC is a late event in skin carcinogenesis, specifically occurring during the transition to spindle cell carcinoma.
    • E-cadherin plays a crucial role in maintaining GJIC in skin epithelial cells.
    • The genetic defect in spindle carcinoma cells involves E-cadherin, while other keratinocyte lines may have different GJIC regulatory mechanisms.