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Related Experiment Videos

Reticulocytes in haematological disorders

G d'Onofrio1, R Kuse, C Foures

  • 1Universita Cattolica, Roma, Italy.

Clinical and Laboratory Haematology
|December 1, 1996
PubMed
Summary

Flow cytometry precisely measures reticulocyte counts and highly fluorescent macroreticulocytes (HFR). This aids in diagnosing hematological disorders by reflecting bone marrow erythrocyte production and erythropoietic stimulation intensity.

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Area of Science:

  • Hematology
  • Clinical Chemistry
  • Flow Cytometry

Background:

  • Flow cytometry offers enhanced precision for reticulocyte count analysis.
  • Measurement of reticulocyte RNA content identifies highly fluorescent macroreticulocytes (HFR), indicating premature release from bone marrow.
  • HFR signifies increased erythropoietic stimulation.

Purpose of the Study:

  • To define reference values for absolute reticulocyte number and HFR percentage in untreated hematological disorders.
  • To utilize Sysmex R-1000 or R-3000 flow cytometers for this analysis.

Main Methods:

  • Analysis of 54 healthy subjects and 100 untreated patients with five hematological diseases.
  • Flow cytometric measurement of reticulocyte count and HFR percentage.

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Main Results:

  • Haemolytic anaemias: Increased reticulocyte count and HFR; inverse correlation between reticulocyte count and Hb.
  • Polycythaemia vera: Moderately increased reticulocytes; direct correlation with Hb.
  • Dyserythropoietic syndromes: Low reticulocytes, moderately increased HFR; inverse correlation between HFR and Hb.
  • Acute myeloid leukaemia: Low reticulocytes, increased HFR; correlation between reticulocytes, HFR, and Hb.
  • Acute lymphoid leukaemia: Normal reticulocyte count, increased HFR.

Conclusions:

  • Established reference values for reticulocyte counts and HFR in hematological diseases.
  • Reticulocyte count reflects effective bone marrow erythrocyte production in anemia.
  • HFR proportion indicates the intensity of erythropoietic stimulation.