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Characterization of an insertion sequence, IS12528, from Gluconobacter suboxydans
1Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Japan.
Applied and Environmental Microbiology
|March 1, 1997
Summary
A novel insertion sequence, IS12528, inactivates alcohol dehydrogenase in Gluconobacter suboxydans by inserting into the adhA gene. This element contributes to genetic instability and physiological deficiencies in acetic acid bacteria.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Acetic acid bacteria, such as Gluconobacter suboxydans, possess complex metabolic pathways.
- Alcohol dehydrogenase (ADH) is crucial for the metabolism of these bacteria.
- Genetic instability can lead to significant alterations in bacterial physiology.
Purpose of the Study:
- To characterize a novel insertion sequence element, IS12528.
- To investigate the role of IS12528 in the inactivation of alcohol dehydrogenase in G. suboxydans.
- To determine the distribution and potential impact of IS12528 in other acetic acid bacteria.
Main Methods:
- Cloning and DNA sequencing of the IS12528 element.
- Analysis of the insertion site and duplication patterns.
- Southern hybridization and PCR for sequence distribution analysis.
- Bioinformatic analysis of the encoded protein sequence.
Main Results:
- IS12528 is a 905 bp insertion sequence with an 18 bp inverted repeat.
- It causes a 3 bp duplication at the insertion site and inactivates the adhA gene.
- IS12528 encodes a putative transposase homologous to the IS1031 family.
- IS12528-like sequences are widespread among various acetic acid bacteria.
Conclusions:
- IS12528 is a mobile genetic element contributing to alcohol dehydrogenase deficiency in G. suboxydans.
- This insertion sequence is a significant factor in the genetic instability of acetic acid bacteria.
- IS12528 may be responsible for various physiological deficiencies observed in these bacteria.