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Rat stromelysin 3: cDNA cloning from healing skin wound, activation by furin and expression in rat tissues
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique/Institut National de la Santé et de la Recherche Médicale/Université Louis Pasteur, Strasbourg, France.
Abstract:
From a rat skin wound healing cDNA library, a clone encoding stromelysin 3 (ST3) was isolated. The predicted rat ST3 has 491 amino acids, and shows 83, 95 and 58% homology with human, mouse and Xenopus ST3, respectively. COS-1 cells transfected with this rat ST3 cDNA produced the ST3 proform, which could be converted to the mature ST3 form by co-transfection with rat furin cDNA. In addition to healing skin, the rat ST3 gene was found to be strongly expressed in normal adult uterus and ovary, and at lower levels in chemically-induced mammary tumors.