Mini review: mitosis and the spindle pole body in Saccharomyces cerevisiae

Cell duplication is characteristic of life. The coordination of cell growth with cell duplication and, specifically, the ordered steps necessary for this process are termed the cell cycle. Central to this process is the faithful replication and segregation of the chromosomes. The cycle consists of four phases: G1, where the decision to enter the cell cycle, which is known as Start, is made; S phase, during which the DNA is replicated; G2, during which controls assuring the completion of S phase operate; and M, or the mitotic phase, which is characterized by chromosome segregation, nuclear division, and cytokinesis. The budding yeast Saccharomyces cerevisiae has been developed into a model genetic system for the study of the cell division cycle (Hartwell et al. ["73] Genetics, 74:267-286). Here I review the basic processes by which chromosomes are segregated, with an emphasis on the physical structures fundamental to this process.