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The Dispatch catheter as a delivery tool for arterial gene transfer
O Tahlil1, M Brami, L J Feldman
1Unité Physiopathologie du Coeur et des Artères, Laboratoire de Pathologie Expérimentale, Faculté Xavier Bichat, Paris, France.
Cardiovascular Research
|January 1, 1997
Summary
This study shows that a new autoperfusion catheter enables efficient arterial gene delivery using adenoviral vectors in normal and diseased arteries. This method avoids ischemia and may improve coronary artery gene therapy.
Area of Science:
- Cardiovascular Research
- Gene Therapy
- Biomedical Engineering
Background:
- Percutaneous arterial gene therapy faces challenges with long incubation times causing ischemia or inefficient transfer in diseased arteries.
- A novel autoperfusion multichamber catheter allows local delivery without myocardial ischemia.
Purpose of the Study:
- To evaluate the efficacy of a new autoperfusion catheter for arterial gene transfer using adenoviral vectors.
- To assess catheter performance in both normal and atheromatous arteries.
Main Methods:
- Replication-defective adenoviral vectors carrying a reporter gene were delivered to iliac arteries of rabbits (normal and atheromatous).
- A multichamber autoperfusion balloon catheter (Dispatch, SciMed) was used for a 60-minute gene delivery duration.
Main Results:
- Efficient reporter gene expression was observed in the endothelium (16% +/- 8%) at the delivery site.
- Low transduction rates were found in medial smooth muscle cells (0.7% +/- 0.4%).
- Transduction in atheromatous arteries occurred in superficial neointima but was lower (1.1% +/- 0.5%); systemic dissemination to the liver was noted in 3/13 animals.
Conclusions:
- Local delivery of adenoviral vectors via the Dispatch autoperfusion catheter achieves efficient gene transfer in endothelial and superficial smooth muscle cells.
- This method is effective in both normal and atheromatous arteries.
- The autoperfusion catheter shows potential as a tool for coronary artery gene therapy.