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Differentiation between bacterial species and sub-species by pyrolysis mass spectrometry of extracted DNA
K Mathers1, R Freeman, P R Sisson
1Department of Microbiology, Medical School, Newcastle-upon-Tyne, UK.
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|January 1, 1997
Abstract:
Whole cell samples from cultures of Staphylococcus aureus, S. hominis, S. epidermidis and Streptococcus pyogenes were analysed by pyrolysis mass spectrometry and the results were compared with Py-MS-derived analyses of samples of DNA extracted from the same organisms. Py-MS analysis differentiated the four organisms in both circumstances. These results challenge previous assumptions that Py-MS is restricted to detecting phenotypic differences, although the basis for the differentiation of the DNA extracts has yet to be determined.