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Conserved structure of IS200 elements in Salmonella

C R Beuzón1, J Casadesús

  • 1Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, E-41080 Sevilla, Spain.

Nucleic Acids Research
|April 1, 1997
PubMed
Summary

Sequence analysis of IS200 elements in Salmonella reveals conserved structures and potential regulatory elements. The study suggests IS200 transposition is unlikely to create inactive copies, indicating low transposition frequency may be normal.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • IS200 is a mobile genetic element found in Salmonella species.
  • Understanding IS200 structure and transposition is crucial for bacterial genetics.

Purpose of the Study:

  • To analyze the conserved structure of IS200 elements.
  • To investigate potential regulatory mechanisms and transposition behavior of IS200.

Main Methods:

  • Sequence analysis of IS200 elements from Salmonella typhimurium and Salmonella abortusovis.
  • Identification of open-reading-frames (ORFs), ribosome-binding sites, and RNA structures.

Main Results:

  • IS200 elements exhibit a conserved structure (707-708 bp) without terminal repeats.

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  • A potential 151-amino acid peptide-encoding ORF, ribosome-binding site, and Rho-independent transcription terminator were identified.
  • IS200 insertion duplicates 1-2 bp of host DNA, and junctions are A+T-rich.

    • Conclusions:

    • IS200 possesses structural features suggesting functional regulation.
    • Transposition of IS200 is unlikely to generate inactive copies, implying low transposition frequency is characteristic behavior.