Characterization of human cyclooxygenase 2 gene promoter localization of a TGF-beta response element

We characterized 2.3 kb of 5'-flanking region of the human cyclooxygenase 2 gene and analyzed its promoter activity. We localized three positive and two negative cis-acting regulatory regions and a TGF-beta response region. Basal promoter activity was located between -77 and +99 bp. Maximal promoter activity was observed from -1838 to +99 bp. A TGF-beta response region was found between -454 and -288 bp. This region included an NF-I site. Electrophoretic mobility shift assays showed that TGF-beta caused a marked change in the pattern and intensity of this NF-I like protein complex.