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Analgesic effects on endogenous melatonin secretion

K R Lapwood1, L Bhagat, M P Simpson

  • 1Department of Physiology and Anatomy, Faculty of Veterinary Science, Massey University, Palmerston North, New Zealand.

Journal of Pineal Research
|January 1, 1997
PubMed
Summary
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Post-operative analgesia is crucial in animal research. Phenylbutazone did not significantly alter melatonin levels in ewes, making it suitable for studies measuring this hormone.

Area of Science:

  • Veterinary Science
  • Pharmacology
  • Endocrinology

Background:

  • Animal welfare necessitates post-operative analgesia in experimental surgery.
  • Analgesic use must not interfere with experimental parameters, such as hormone levels.
  • Melatonin, a key hormone, can be influenced by various treatments.

Purpose of the Study:

  • To evaluate the effect of common analgesics on endogenous melatonin production in ewes.
  • To identify a suitable post-operative analgesic that does not affect melatonin secretion for future research.

Main Methods:

  • Groups of six ewes were used for the study.
  • Blood samples were collected at 30-minute intervals for 4 hours during darkness.
  • Plasma melatonin levels were measured using radioimmunoassay (RIA) following administration of ketoprofen, phenylbutazone, or buprenorphine.

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Main Results:

  • Ketoprofen and buprenorphine significantly reduced mean 4-hour melatonin secretion by 50.6% and 38.6%, respectively.
  • Phenylbutazone administration did not result in a statistically significant change in melatonin secretion.
  • Individual melatonin secretion profiles were computed for each ewe.

Conclusions:

  • Ketoprofen and buprenorphine interfere with melatonin production in ewes.
  • Phenylbutazone is a suitable post-operative analgesic for experimental studies involving melatonin measurement in ewes.
  • Careful selection of analgesics is essential to maintain the validity of experimental parameters in animal research.