Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in protein composition during chick embryo retina development

M Carabillò1, E Gerbino, H Main

  • 1Istituto di Chimica Biologica, Università of Palermo, Policlinico, Italy.

Biochemistry and Molecular Biology International
|February 1, 1997
PubMed
Summary

This study analyzed chick embryo retina proteins during development using two-dimensional gel electrophoresis. Key protein changes were identified, aiding in understanding retina growth and differentiation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Expression of Concern] Histone deacetylase inhibitors induce in human hepatoma HepG2 cells acetylation of p53 and histones in correlation with apoptotic effects.

International journal of oncology·2026
Same author

Fortification of water kefir with magnetite nanoparticles.

Food research international (Ottawa, Ont.)·2021
Same author

Development and characterization of iron-pectin beads as a novel system for iron delivery to intestinal cells.

Colloids and surfaces. B, Biointerfaces·2018
Same author

Valorization of okara oil for the encapsulation of Lactobacillus plantarum.

Food research international (Ottawa, Ont.)·2018
Same author

Coheredity of a new silent mutation: c.-29G>T, with a severe β-thal mutation in a patient with β-thalassemia intermediate.

International journal of laboratory hematology·2018
Same author

New Codanin-1 Gene Mutations in a Italian Patient with Congenital Dyserythropoietic Anemia Type I and Heterozygous Beta-Thalassemia.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion·2016

Area of Science:

  • Developmental Biology
  • Proteomics
  • Neuroscience

Background:

  • Understanding the molecular mechanisms of embryonic development is crucial.
  • The chick embryo retina serves as a model for studying neural development.
  • Protein expression patterns change significantly during tissue differentiation.

Purpose of the Study:

  • To analyze the protein composition of chick embryo retina during
  • in ovo
  • development.
  • To identify specific protein components characteristic of different developmental stages.
  • To investigate changes in protein expression during retina maturation.

Main Methods:

  • Two-dimensional gel electrophoresis (2DGE) was used to analyze soluble proteins.

Related Experiment Videos

  • Preparative isoelectric focusing was employed for partial purification of a specific protein.
  • Comparative 2DGE was performed on liver and brain tissues at different developmental days.

    • Main Results:

    • Approximately eighty soluble protein components were identified in 7-day-old chick embryo retinas.
    • Four proteins showed decreased staining intensity during retina development.
    • A 35 kDa protein with an isoelectric point of 5.15 was partially purified.
    • New protein components appeared during the second week of development, visible by day 16.

    Conclusions:

    • Protein expression in the developing chick embryo retina is dynamic.
    • Specific protein profiles can serve as markers for different stages of retinal development.
    • These findings contribute to the understanding of molecular events in neural tissue differentiation.