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DNA sequence and nucleosome placement on the murine fibroblast growth factor-4 gene
P J Wilder1, C Mountjoy, M C Macleod
1Eppley Institute for Cancer Research, UNMC/Eppley Cancer Center, University of Nebraska Medical Center, Omaha 68198-6805, USA.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1997
Abstract:
A large portion of the 5' flanking region of the fibroblast growth factor-4 (FGF-4) gene has been sequenced, but only 38 bp of sequence information past the end of the last exon of this gene has been described. In this study, a total of 2769 bp of nucleotide sequence down-stream of the last exon of FGF-4 (GenBank #U43515) was determined by a combination of deletional subcloning and primer walking. In addition, we have used the theoretical algorithm of Calladine and Drew to predict the rotational positioning of nucleosomes throughout the entire FGF-4 gene.