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Immunohistochemical study of hepatocellular carcinoma-specific aldehyde dehydrogenase
A Shibuya1, A Takeuchi, H Shibata
1Department of Internal Medicine, Kitasato University, School of Medicine, Sagamihara, Kanagawa, Japan.
Abstract:
Tumor-associated aldehyde dehydrogenase (ALDH) was reported in cases of human hepatocellular carcinoma and animal hepatoma models. This ALDH isozyme is similar to ALDH3 which exists in the stomach and lung; however, the biochemical and clinical significance of this unique ALDH isozyme have not been established. Human tumor-associated ALDH was purified, and polyclonal antibodies prepared. Using these antibodies, specific development of tumor-associated ALDH was confirmed by immunohistochemical techniques. It was found that about 50% of hepatocellular carcinomas reacted with the antibody. This unique ALDH isozyme may be a novel tumor marker of hepatocellular carcinoma.