[The binding and absorption by mollicute cells of antisignature analogs of oligodeoxyribonucleotides]

Processes of absorption and uptake of phosphorothioate, phosphorodithioate and methylphosphonate analogs of oligodeoxynucleotides which are complementary to "signature" 16S rRNA sequences by cells of Acholeplasma laidlawii PG-8. Mycoplasma fermentans PG-18 and Mycoplasma pneumoniae FH have been investigated. Distinctions in efficiency of absorption by given cells of phosphorothioate and methylphosphonate analogs of oligonucleotides are found out. So, the level of thioates sorption by the cells of A. laidlawii PG-8 exceeded a share of methylphosphonates binding with these cells 5 times as high. A fact of significant accumulation of thio- and dithioate analogs of oligonucleotides by the mollicute cells is established. The intracellular concentration of these compounds in all investigated microorganisms exceeded extracellular one 10(4)-10(5) time. On the basis of obtained experimental data the assumption is made on existence in mollicute cells of two various mechanisms of uptake of analogs of oligonucleotides bearing a negative charge in sugar-phosphate backbone and nonionic oligonucleotide analogs.