Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calmodulin regulates endosome fusion

M I Colombo1, W Beron, P D Stahl

  • 1Department of Cell Biology and Physiology, School of Medicine, Washington University, St. Louis, Missouri 63110, USA.

The Journal of Biological Chemistry
|March 21, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The cell biology of Extracellular Vesicles: A jigsaw puzzle with a myriad of pieces.

Current opinion in cell biology·2025
Same author

Ursolic acid: A novel antiviral compound inhibiting rotavirus infection in vitro.

International journal of antimicrobial agents·2019
Same author

SPARC (secreted protein acidic and rich in cysteine) knockdown protects mice from acute liver injury by reducing vascular endothelial cell damage.

Gene therapy·2014
Same author

Autophagy and toxins: a matter of life or death.

Current molecular medicine·2012
Same author

A membrane is born: origin of the autophagosomal compartment.

Current molecular medicine·2011
Same author

Coxiella burnetii modulates Beclin 1 and Bcl-2, preventing host cell apoptosis to generate a persistent bacterial infection.

Cell death and differentiation·2009
Same journal

Wanted and unwanted modifications of mRNA, and their effect on gene expression and signaling.

The Journal of biological chemistry·2026
Same journal

TGF-β2 drives lipid droplet accumulation in chondrocytes through the TβRI/p-Smad3/Fabp5 axis.

The Journal of biological chemistry·2026
Same journal

Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivo.

The Journal of biological chemistry·2026
Same journal

Substrate and target selectivity of 4'-fluoroadenosine against viral and host polymerases.

The Journal of biological chemistry·2026
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
See all related articles

Calmodulin (CaM) regulates endosomal membrane trafficking by influencing endosome fusion. CaM antagonists inhibit this process, but CaM addition restores fusion, suggesting a key role for CaM in endocytic transport.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Membrane Trafficking

Background:

  • Calmodulin (CaM) is known to be involved in various cellular processes including exocytosis, transcytosis, and receptor recycling.
  • The specific role of CaM in endocytic transport and endosomal membrane trafficking remains less understood.
  • Investigating CaM's function in endocytosis is crucial for understanding cellular cargo processing and sorting.

Purpose of the Study:

  • To investigate the role of Calmodulin (CaM) in endocytic transport and endosomal membrane trafficking.
  • To determine the specific mechanisms by which CaM influences endosomal fusion.
  • To identify potential molecular targets of CaM involved in regulating endocytic pathways.

Main Methods:

  • Utilized CaM antagonists in intact cells to observe effects on endocytic transport.

Related Experiment Videos

  • Assessed the mixing of sequentially internalized ligands to evaluate endosomal trafficking.
  • Employed an in vitro assay to reconstitute and study endosome fusion dynamics.
  • Investigated the effects of Ca2+ and CaM on endosome fusion in vitro.

    • Main Results:

    • CaM antagonists inhibited the mixing of sequentially internalized ligands, indicating a role for CaM in endosomal membrane trafficking.
    • In vitro assays showed that CaM antagonists inhibit endosome fusion, an effect reversed by CaM addition.
    • Both Ca2+ and CaM stimulated endosome fusion, with CaM providing an additional stimulatory effect beyond Ca2+ alone.
    • CaM-dependent kinase II was identified as a potential target for CaM in the context of endosome fusion.

    Conclusions:

    • Calmodulin (CaM) plays a significant role in regulating endocytic transport, particularly endosomal membrane trafficking and fusion.
    • CaM appears to modulate essential components of the membrane traffic machinery, potentially via CaM-dependent kinase II.
    • These findings highlight CaM as a key regulator of endosomal fusion and overall endocytic pathway function.