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Identification of two palmitoyl groups in octopus rhodopsin
M Nakagawa1, T Iwasa, S Kikkawa
1Department of Life Science, Himeji Institute of Technology, Hyogo, Japan.
Photochemistry and Photobiology
|January 1, 1997
Abstract:
We determined the structure and site of fatty acid incorporated in octopus rhodopsin using a combination of fluorescence label and enzymatic cleavage methods in conjunction with fast-atom bombardment (FAB) mass spectrometry. A single peptide containing two adjacent cysteines, Cys337 and Cys338, was successfully isolated using the fluorescence from a dye conjugated to Cys345. The FAB mass spectrometric analysis of the peptide (323Phe-340Phe) revealed that two palmitoyl groups are linked to Cys337 and Cys338 via thioester bonds in octopus rhodopsin as in bovine rhodopsin.