Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequential bilateral isolated lung perfusion in the rat: an experimental model

S Nawata1, A Abolhoda, H M Ross

  • 1Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.

The Annals of Thoracic Surgery
|March 1, 1997
PubMed
Summary

Sequential bilateral isolated lung perfusion is a safe and well-tolerated model in rats for studying staged chemotherapeutic treatments for pulmonary metastases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cancer Council's Tackling Tobacco program: an Australian initative building partnerships for public health research impact.

Public health research & practice·2026
Same author

Risks to the future health and productivity of tropical estuaries under climate change and increasing human development.

Marine pollution bulletin·2025
Same author

Two decades of recipient and donor referrals for heart transplantation to Groote Schuur Hospital, Cape Town, South Africa: A retrospective study.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024
Same author

Common Arterial Trunk Repair at the Red Cross War Memorial Hospital, Cape Town: A 20-Year Review of Surgical Practice and Outcomes.

World journal for pediatric & congenital heart surgery·2024
Same author

Sustainable financing for Immunization Agenda 2030.

Vaccine·2022
Same author

Better Understanding of the Metamorphosis of Pregnancy (BUMP): protocol for a digital feasibility study in women from preconception to postpartum.

NPJ digital medicine·2022

Area of Science:

  • Thoracic Surgery
  • Pulmonary Medicine
  • Surgical Models

Background:

  • Established a rat model for isolated single-lung perfusion to study chemotherapy for pulmonary metastases.
  • Designed a sequential bilateral isolated lung perfusion model to assess staged perfusions in rats.

Purpose of the Study:

  • To investigate the feasibility and safety of sequential bilateral isolated lung perfusion in a rat model.
  • To establish a reliable model for future studies on chemotherapeutic agents for bilateral pulmonary metastases.

Main Methods:

  • Twenty-four Fischer rats were divided into three experimental groups (n=8) undergoing left isolated lung perfusion, followed by contralateral perfusion at 1, 2, or 3 weeks.
  • A control group (n=5) underwent sequential bilateral sham thoracotomies.

Related Experiment Videos

  • Arterial blood gas analysis was performed post-second operation in one experimental group and the control group.

    • Main Results:

    • Survival rates for the second operation were 100% (Group I), 75% (Group II), and 100% (Group III). All control animals survived.
    • Arterial blood gas analysis showed no significant differences in oxygen, carbon dioxide, or pH between the experimental and control groups.

    Conclusions:

    • Sequential bilateral isolated lung perfusion is safe and well-tolerated in rats.
    • This model is suitable for evaluating the safety and efficacy of staged perfusions for treating bilateral pulmonary metastases.