Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Monastral bipolar spindles: implications for dynamic centrosome organization

P G Wilson1, M T Fuller, G G Borisy

  • 1Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA.

Journal of Cell Science
|February 1, 1997
PubMed
Summary

Mutations in the urchin gene create functional monastral bipolar spindles, challenging the view of permanent centrosomes. This suggests motor proteins influence dynamic centrosome organization during cell division.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improved Visualization of Oral Microbial Consortia.

Journal of dental research·2024
Same author

A decade of day-case endoscopically guided stent placement in malignant oesophagogastric strictures.

Surgical endoscopy·2016
Same author

Myrtaceae revisited: a reassessment of infrafamilial groups.

American journal of botany·2011
Same author

Presentations of pyogenic liver abscess in one UK centre over a 15-year period.

The journal of the Royal College of Physicians of Edinburgh·2011
Same author

An unusual case of gastric erosions.

Gut·2009
Same author

Investigation of secondary structures and macromolecular interactions in bacteriophage p22 by laser Raman spectroscopy.

Biophysical journal·2009

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Centrosomes are traditionally considered permanent structures essential for mitotic spindle assembly.
  • The urchin gene's role in centrosome organization and spindle bipolarity was previously unclear.

Purpose of the Study:

  • To investigate the function of monastral bipolar spindles in urchin mutants.
  • To determine if centrosome structure is dynamic during mitosis.

Main Methods:

  • Immunofluorescence microscopy of larval brains from Drosophila urchin mutants.
  • Pedigree analysis of centrosome distribution and spindle structure in gonial cell divisions.
  • Allelic testing of urchin with KLP61F.

Main Results:

Related Experiment Videos

  • Urchin mutants frequently displayed monastral bipolar spindles, with one centrosome at the astral pole.
  • Despite monastral spindles, mitotic divisions yielded cells of equivalent size and chromatin content.
  • Cells derived from the anastral pole could form functional bipolar spindles in subsequent cycles.

Conclusions:

  • Monastral bipolar spindles are functional, indicating a dynamic nature of centrosome organization.
  • Motor proteins, like the one encoded by KLP61F, likely influence centrosome dynamics.