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Adenosine activates mesangial cell proliferation

M MacLaughlin1, C Martinez-Salgado, N Eleno

  • 1Instituto Reina Sofía de Investigacíon Nefrológica, Departamento de Fisiología y Farmacología, Universidad de Salamanca, Spain.

Cellular Signalling
|January 1, 1997
PubMed
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Adenosine (ADO) stimulates proliferation in cultured mesangial cells. This effect is mediated by ADO receptors but is not specific to either A1 or A2 subtypes.

Area of Science:

  • Nephrology
  • Cell Biology
  • Pharmacology

Background:

  • Adenosine (ADO) is a nucleoside with diverse physiological roles.
  • Mesangial cells are crucial components of the kidney glomerulus.
  • The role of adenosine receptors in mesangial cell proliferation requires further elucidation.

Purpose of the Study:

  • To investigate the proliferative effect of adenosine on cultured mesangial cells.
  • To determine the involvement of A1 and A2 adenosine receptors in this proliferative response.

Main Methods:

  • Cultured human mesangial cells were treated with adenosine (ADO) and specific receptor agonists/antagonists.
  • [3H]thymidine incorporation was measured to assess DNA synthesis and cellular proliferation.
  • Theophylline, an ADO receptor inhibitor, was used to block ADO-induced proliferation.

Related Experiment Videos

Main Results:

  • Adenosine (10(-5) M) significantly increased [3H]thymidine incorporation in mesangial cells.
  • Both A1 and A2 receptor agonists (R-PIA and NECA, respectively) mimicked this proliferative effect.
  • Theophylline completely inhibited adenosine-induced proliferation, confirming receptor mediation.
  • Combinations of agonists with specific antagonists did not reveal a subtype-specific effect.

Conclusions:

  • Adenosine exerts a proliferative effect on cultured mesangial cells.
  • This proliferative effect is mediated through adenosine receptors.
  • The study suggests that neither A1 nor A2 receptor activation is solely responsible for adenosine-induced mesangial cell proliferation.