Thermal cycle dideoxy DNA sequencing
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Updated: Nov 2, 2026

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
Published on: June 26, 2016
1New England Biolabs, Inc., Beverly, MA 01915, USA.
Abstract:
Thermal cycle dideoxy DNA sequencing eliminates the requirements for independent primer annealing and double-stranded DNA denaturation steps. The method enables sequencing from nanogram amounts of DNA from double-stranded and single-stranded PCR products, and plasmid or phage DNA templates. Thermal cycle sequencing also enables direct sequencing from bacterial colonies or phage plaques. Protocols using the Vent exo- DNA polymerase, helpful suggestions, and a troubleshooting guide are also presented.
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