Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thermal cycle dideoxy DNA sequencing

B E Slatko1

  • 1New England Biolabs, Inc., Beverly, MA 01915, USA.

Molecular Biotechnology
|December 1, 1996
PubMed
Summary
This summary is machine-generated.

Thermal cycle dideoxy DNA sequencing simplifies DNA analysis by removing separate primer annealing and denaturation steps. This method allows efficient DNA sequencing from various sources, including PCR products and bacterial colonies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Filarial and Wolbachia genomics.

Parasite immunology·2011
Same author

Denaturing polyacrylamide gel electrophoresis.

Current protocols in human genetics·2008
Same author

Denaturing polyacrylamide gel electrophoresis.

Current protocols in nucleic acid chemistry·2008
Same author

Preparation of templates for DNA sequencing.

Current protocols in molecular biology·2008
Same author

DNA sequencing by the dideoxy method.

Current protocols in molecular biology·2008
Same author

Denaturing gel electrophoresis for sequencing.

Current protocols in molecular biology·2008
Same journal

Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65.

Molecular biotechnology·2026
Same journal

Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.

Molecular biotechnology·2026
Same journal

CRISPR/Cas9 Mediated Genome Editing for Enhancing Abiotic Stress Tolerance in Rice: An Omics Guided Perspective.

Molecular biotechnology·2026
Same journal

Functionalization of Chitosan with Silver Oxide and Berberis aristata for Bioactive Composites with In Vivo Wound Healing Evaluation.

Molecular biotechnology·2026
Same journal

Resilience in Tribolium Castaneum Through Antioxidant Priming Under Phosphine-Induced Oxidative Stress.

Molecular biotechnology·2026
Same journal

MicroRNA-181a-5p promotes papillary thyroid carcinoma progress via the PTEN/AKT pathway.

Molecular biotechnology·2026
See all related articles

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Traditional dideoxy DNA sequencing involves multiple steps, including primer annealing and DNA denaturation.
  • These steps can be time-consuming and require specific conditions, limiting throughput and accessibility.

Purpose of the Study:

  • To introduce and describe a simplified thermal cycle dideoxy DNA sequencing method.
  • To demonstrate the method's efficiency with various DNA templates and direct sequencing applications.

Main Methods:

  • Developed a thermal cycling protocol for dideoxy DNA sequencing, eliminating separate denaturation and annealing steps.
  • Utilized Vent exo- DNA polymerase for the sequencing reactions.
  • Applied the method to double-stranded and single-stranded PCR products, plasmid DNA, and phage DNA templates.

Related Experiment Videos

Main Results:

  • Successfully sequenced DNA from nanogram amounts using the thermal cycle method.
  • Enabled direct sequencing from bacterial colonies and phage plaques.
  • Provided detailed protocols, helpful suggestions, and a troubleshooting guide for the method.

Conclusions:

  • Thermal cycle dideoxy DNA sequencing offers a streamlined and efficient approach to DNA analysis.
  • The method is versatile, accommodating various DNA sources and direct sequencing applications.
  • The presented protocols and guidance facilitate the adoption and troubleshooting of this technique.