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Stem cell factor stimulates chicken osteoclast activity in vitro

R J van't Hof1, M von Lindern, P J Nijweide

  • 1Department of Cell Biology, University of Leiden, The Netherlands.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|March 1, 1997
PubMed
Summary

Stem cell factor (SCF) significantly boosts chicken osteoclast formation and bone resorption. Osteoblasts express SCF, with parathyroid hormone increasing its production, suggesting a key role in bone remodeling.

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Area of Science:

  • Cell Biology
  • Bone Biology
  • Hematopoiesis

Background:

  • Stem cell factor (SCF) is a growth factor influencing hematopoietic cells.
  • Osteoclasts are crucial for bone resorption, and their regulation is vital for bone health.

Purpose of the Study:

  • To investigate the effects of avian stem cell factor (SCF) on chicken osteoclast differentiation and bone-resorbing activity.
  • To determine if chicken osteoblasts express and secrete SCF and how its expression is regulated.

Main Methods:

  • Osteoclast differentiation was assessed using long-term co-cultures of osteoblasts and bone marrow cells.
  • Bone-resorbing activity was measured in short-term cultures of osteoclast-enriched populations on dentine slices.
  • SCF expression in osteoblasts was analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR).

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Main Results:

  • SCF significantly enhanced osteoclast differentiation, with additive effects when combined with chicken myelomonocytic growth factor (cMGF).
  • SCF, but not cMGF, increased the bone-resorbing activity of mature osteoclasts.
  • Chicken osteoblasts express SCF mRNA, and parathyroid hormone increases this expression approximately fourfold; SCF remains cell-associated.

Conclusions:

  • Avian SCF plays a significant role in both the formation and function of chicken osteoclasts.
  • Osteoblasts are a source of SCF, and its expression is regulated by parathyroid hormone, indicating a potential mechanism for controlling bone remodeling.