Ca(2+)-dependency of spinule plasticity at dendrites of retinal horizontal cells and its possible implication for the...

Calcium is involved in many aspects of synaptic plasticity and we have analyzed its involvement in spinule dynamics at retinal horizontal cell dendrites. We show here that in particular the retraction of spinules is a Ca(2+)-dependent process. Inhibiting calmodulin or CaMKII, blocked the retraction that was also impaired in low calcium Ringer. Changes of the cytosolic Ca(2+)-concentration through depletion of internal Ca(2+)-stores were without effect. This suggested that Ca(2+)-influx during dark adaption and subsequent activation of CaMKII is an important step for spinule retraction. Voltage dependent Ca(2+)-channels were not responsible for the Ca(2+)-influx, rather Ca2+ leaking through alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate-gated channels. This suggested a close local link between AMPA/kainate receptors and CaMKII indicating a possible postsynaptic function of spinules. The distribution of bound, omega-shaped vesicles within the cone pedicles and its dependence on artificial depolarization further supported the idea of a postsynaptic function of spinules.