Selection of sputum specimens for microbiological examination
Related Experiment Video
Updated: Nov 2, 2026
Methodology for Sputum Induction and Laboratory Processing
Published on: December 17, 2017
1Department of Medical Microbiology, Rotherham General Hospital NHS Trust, England, UK.
Abstract:
The purpose of this study was to compare the leucocyte esterase (LE) strip test with direct microscopy of Gram-stained sputum smears for the demonstration of leucocytes (WBC) and to develop a simple method for selecting specimens suitable for cultural examination. A total of 11% of specimens were demonstrated to be unsuitable for bacteriological examination using the proposed algorithm. It is thought that this technique offers a simple alternative to traditional microscopy alone for screening sputum specimens.
Related Concept Videos
01:26Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
01:20Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...