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Interleukin-13 inhibits cytokine secretion by blood monocytes from patients with IgA nephropathy
1Second Department of Internal Medicine, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan.
In this study we have examined the effects of recombinant human interleukin (IL)-13 on peripheral blood monocytes (PBM) from patients with IgA nephropathy (IgAN). Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated secretion of tumor necrosis factor-alpha (TNF) and IL-8 was determined in PBM cultures of IgAN patients compared to those of normal controls. In the present study, IL-13 inhibited the spontaneous as well as the LPS-stimulated cytokine secretion of PBM in IgAN. Significant inhibitory effect of IL-13 was observed in cultures of PBM from IgAN patients as well as from normal persons. When both LPS and anti-IL-13 antibody were added together to the PBM, a further increase of LPS-enhanced secretion of cytokines occurred. Taken together, these results indicate that IL-13 down-regulates the secretion of TNF and IL-8 from IgAN PBM.
In this study we have examined the effects of recombinant human interleukin (IL)-13 on peripheral blood monocytes (PBM) from patients with IgA nephropathy (IgAN). Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated secretion of tumor necrosis factor-alpha (TNF) and IL-8 was determined in PBM cultures of IgAN patients compared to those of normal controls. In the present study, IL-13 inhibited the spontaneous as well as the LPS-stimulated cytokine secretion of PBM in IgAN. Significant inhibitory effect of IL-13 was observed in cultures of PBM from IgAN patients as well as from normal persons. When both LPS and anti-IL-13 antibody were added together to the PBM, a further increase of LPS-enhanced secretion of cytokines occurred. Taken together, these results indicate that IL-13 down-regulates the secretion of TNF and IL-8 from IgAN PBM.