Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for preparing ferruginous bodies for electron microscopic examination

A Churg, N Sakoda, M L Warnock

    American Journal of Clinical Pathology
    |October 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    New Insights on Diagnostic Reproducibility of Biphasic Mesotheliomas: A Multi-Institutional Evaluation by the International Mesothelioma Panel From the MESOPATH Reference Center.

    Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer·2018
    Same author

    Pathogenesis of Fibrosis Produced by Asbestos and Man-Made Mineral Fibers: What Makes a Fiber Fibrogenic?

    Inhalation toxicology·2015
    Same author

    Diagnosing organizing pneumonia: Limitations of radiology and pathology.

    Revista clinica espanola·2014
    Same author

    Evanescent wave coupling in terahertz waveguide arrays.

    Optics express·2013
    Same author

    Pulmonary hypertension and vascular oxidative damage in cigarette smoke exposed eNOS(-/-) mice and human smokers.

    Inhalation toxicology·2012
    Same author

    Series "matrix metalloproteinases in lung health and disease": Matrix metalloproteinases in COPD.

    The European respiratory journal·2011

    A novel method simplifies preparing ferruginous (asbestos) bodies from lung tissue for electron microscopy. This technique enhances detection of asbestos bodies, even in minimally exposed individuals, aiding in accurate diagnosis.

    Area of Science:

    • Pulmonary pathology
    • Materials science
    • Electron microscopy

    Background:

    • Ferruginous (asbestos) bodies are indicators of asbestos exposure.
    • Accurate detection and characterization are crucial for health assessments.
    • Existing methods can be challenging, especially with low body counts.

    Purpose of the Study:

    • To introduce a new, efficient method for preparing ferruginous bodies for electron-optical examination.
    • To improve the detection and analysis of ferruginous bodies in pulmonary tissue.

    Main Methods:

    • Pulmonary tissue is digested using bleach.
    • Residue containing ferruginous bodies is collected on a Millipore filter.
    • Filter sections with bodies are processed for electron microscopy using acetone vapor to dissolve the filter.

    Related Experiment Videos

    Main Results:

    • The method successfully isolates ferruginous bodies and submicroscopic fibers on electron microscope grids.
    • It is effective for samples with varying levels of asbestos exposure, particularly those with few bodies.
    • Facilitates easy identification of body cores via electron diffraction and microprobe analysis.

    Conclusions:

    • This technique offers a simplified and effective approach for preparing ferruginous bodies for detailed electron-optical analysis.
    • It enhances the ability to diagnose asbestos exposure, even in cases with minimal exposure.
    • The method supports precise characterization of asbestos body cores.