Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane assembly studied by spin-label electron spin resonance

D Marsh1

  • 1Max-Planck-Institut für biophysikalische Chemie, Abteilung Spektroskopie, Göttingen, Germany.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|July 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Departmental audit on immobilisation in a small cohort of patients with lower limb sarcoma.

Radiography (London, England : 1995)·2025
Same author

A global call to action to improve the care of people with fragility fractures.

Injury·2018
Same author

Low-intensity pulsed ultrasound does not influence bone healing by distraction osteogenesis: a multicentre double-blind randomised control trial.

The bone & joint journal·2017
Same author

EULAR/EFORT recommendations for management of patients older than 50 years with a fragility fracture and prevention of subsequent fractures.

Annals of the rheumatic diseases·2016
Same author

FRAX-based assessment and intervention thresholds--an exploration of thresholds in women aged 50 years and older in the UK.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
Same author

Local motion and conformational changes in the cuticle of Clivia miniata Regel : A microfluorescence and spin-label study.

Planta·2013

Electron spin resonance (ESR) studies reveal how lipids interact with membrane proteins and how proteins move within membranes. Findings show lipid mobility is affected by protein interactions, influencing protein assembly and function.

Area of Science:

  • Biophysics
  • Membrane Biology
  • Spectroscopy

Background:

  • Integral membrane proteins play crucial roles in cellular functions.
  • Understanding lipid-protein interactions is key to deciphering membrane dynamics and protein function.

Purpose of the Study:

  • To investigate lipid-protein interactions in biological membranes using conventional electron spin resonance (ESR).
  • To analyze the mobility and aggregation states of integral membrane proteins via saturation transfer ESR.
  • To explore new ESR-based methods for determining lipid exchange, protein diffusion, and protein localization.

Main Methods:

  • Conventional ESR of spin-labeled lipids to study lipid-protein interactions.
  • Saturation transfer ESR of spin-labeled proteins to assess protein mobility and aggregation.

Related Experiment Videos

  • Spectral analysis including subtraction, addition, and simulation with exchange-coupled Bloch equations.
  • ESR power saturation properties to determine lipid exchange and protein diffusion rates.

    • Main Results:

    • Two distinct lipid populations were identified, with one showing hindered mobility due to protein interaction.
    • Lipid exchange rates at the protein interface were consistent with other methods and reflected lipid selectivity.
    • Integral protein rotation was found to be primarily uniaxial, with anisotropic motion analysis providing insights into protein assembly.
    • New ESR techniques were employed to quantify protein translational diffusion and membrane penetration.

    Conclusions:

    • ESR spectroscopy is a powerful tool for elucidating lipid-protein interactions and membrane protein dynamics.
    • Lipid-protein interactions significantly influence lipid mobility and membrane protein behavior.
    • ESR-based methods offer versatile approaches to study complex membrane systems and protein assembly.